Use Of Chemometrics For Development And Validation Of RP-HPLC Method For Simultaneous Determination Of Dimenhydrinate And Cinnarizine

A novel simple, sensitive, rapid and accurate isocratic RP-HPLC method was developed validated for simultaneous determination of Dimenhydrinate Cinnarizine applying statistical experimental design. Design experiments (DoE) applied multivariate optimization the conditions method. Three independent factors like; mobile phase composition, phosphate buffer strength flow rate were employed to design mathematical models. Central composite (CCD) study response surface methodology. This used find deepness effects these factors. Desirability function simultaneously optimize retention time resolution analytes. Hence, our present aimed in finding optimized predicted data from contour diagram. Mobile consisted acetonitrile ammonium acetate (pH 7.0, 0.3 mM) ratio 55:45%v/v respectively, at a 1.7 mL/min used. The objectives apply optimum baseline partition both drugs with good resolution. run less than 12.0 min accomplished. assay according ICH guidelines. correlation coefficient value found be r2 = 0.9990 0.9993 respectively. LOD LOQ least concentration drugs. It indicates highly sensitive. accuracy %RSD 2%. Consequently reports clearly showed that Quality by approach could triumphantly Cinnarizine.